Cover Art Gallery


Michael Nash on Google Scholar

#Equally contributing authors; *Corresponding author

Santos, M. S., Liu, H., Schittny, V., Vanella, R., and Nash, M. A.*
Correlating Single-molecule Rupture Mechanics with Cell Population Adhesion by Yeast Display
Biophysical Reports, 2(1), 10035, 2022.

Associated Data


Vanella, R., Kovacevic, G. N., Doffini, V., Santaella, J. F., and Nash, M. A.*
High-throughput Screening, Next Generation Sequencing and Machine Learning: Advanced Methods in Enzyme Engineering
Chemical Communications, 58: 2455–2467, 2022.


Nash, M. A.*
Scalable Online Learning in Physical Chemistry 
Chimia, 75: 64–66, 2021. 


Zhaowei Liu, Rodrigo A. Moreira, Ana Dujmović, Haipei Liu, Byeongseon Yang, Adolfo B. Poma, Michael A. Nash*
Mapping Mechanostable Pulling Geometries of a Therapeutic Anticalin/CTLA-4 Protein Complex
Nano Letters 22(1): 179–187, 2021. bioRxiv 2021.03.09.434559



Huo, Z., Santos, M. S., Drenckhan, A., Holland-Cunz, S., Izbicki, J. R., Nash, M. A., and Gros, S. J.
Metastatic Esophageal Carcinoma Cells Exhibit Reduced Adhesion Strength and Enhanced Thermogenesis
Cells 10(5), 1213; DOI: 10.3390/cells10051213, 2021


Yang, B., Liu, H., Liu, Z., Doenen, R., and Nash, M. A.*
Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex
Nano Letters, DOI: 10.1021/acs.nanolett.0c04178; BioRxiv 10.1101/2020.07.09.194894v1, 2020
Associated Data


Urosev, I., Morales, J. L., and Nash, M. A.*
Phase Separation of Intrinsically Disordered Protein-polymers Mechanically Stiffens Fibrin Clots
Advanced Functional Materials
, 2020, 2005245; DOI: 10.1002/adfm.202005245


Liu, Z., Liu, H., Vera, A. M., Bernardi, R. C., Tinnefeld, P., and Nash, M. A.*
High Force Catch Bond Mechanism of Bacterial Adhesion in the Human Gut
re Communications
11, 4321 (2020),


Nash, M. A.*
Single-molecule and Single-cell Approaches in Molecular Bioengineering
74:704–709, 2020


Jensen, M. H., Morris, E. J., Tran, H., Nash, M. A., and Tan, C.
Stochastic Ordering of Complexoform Protein Assembly by Genetic Circuits
PLOS Computational Biology, DOI: 10.1371/journal.pcbi.1007997, 2020


Yang, B., Liu, Z., Liu, H., and Nash, M. A.*
Next Generation Methods for Single-molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes
Frontiers in Molecular Biosciences DOI: 10.3389/fmolb.2020.00085, 2020


Nash, M. A.*
Zig Zag AFM Protocol Reveals New Intermediate Folding States of Bacteriorhodopsin
Biophysical Journal
, 118(3): 538-540, 2020


Bernardi, R. C.*#, Durner, E.#, Schoeler, C., Malinowska, K. H., Carvalho, B. G., Bayer, E. A., Luthey- Schulten, Z., Gaub, H. E., and Nash, M. A.*
Mechanisms of Nanonewton Mechanostability in a Protein Complex Revealed by Molecular Dynamics Simulations and Single-Molecule Force Spectroscopy
Journal of the American Chemical Society
, 141(37): 14752-14763, 2019


Liu, H., Schittny, V., and Nash, M. A.*
Removal of a Conserved Disulfide Bond Does Not Compromise Mechanical Stability of a VHH Antibody Complex
Nano Letters, 19(8): 5524-5529, 2019


Vanella, R., Ta, D. T., and Nash, M. A.*
Enzyme‐mediated hydrogel encapsulation of single cells for high‐throughput screening and directed evolution of oxidoreductases
Biotechnology and Bioengineering, 116(8): 1878–1886, 2019


Vanella, R., Bazin, A., Ta, D. T., and Nash, M. A.*
Genetically Encoded Stimuli-Responsive Cytoprotective Hydrogel Capsules for Single Cells Provide Novel Genotype−Phenotype Linkage
Chemistry of Materials, 31(6): 1899–1907, 2019


Lewis, D. D., Vanella, R., Vo, C., Rose, L., Nash, M. A., and Tan, C.
Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress
Cellular and Molecular Bioengineering, 11(5): 367-382, 2018


Ta, D. T., Vanella, R., and Nash, M. A.*
Bioorthogonal Elastin-like Polypeptide Scaffolds for Immunoassay Enhancement
ACS Applied Materials & Interfaces, 10(36): 30147-30154, 2018


Gunnoo, M., Cazade, P. A., Orlowski, A., Chwastyk, M., Liu, H., Ta, D. T., Cieplak, M., Nash, M. A., and Thompson, D.
Steered Molecular Dynamics Simulations Reveal the Role of Ca2+ in Regulating Mechanostability of Cellulose-binding Proteins
Physical Chemistry Chemical Physics, 20(35): 22674-22680, 2018


Ta, D. T., Vanella, R., and Nash, M. A.*
Magnetic Separation of Elastin-like Polypeptide Receptors for Enrichment of Cellular and Molecular Targets
Nano Letters, 17(12): 7932-7939, 2017


Verdorfer, T., Bernardi, R., Meinhold, A., Ott, W., Luthey-Schulten, Z., Nash, M. A.*, and Gaub. H. E.
Combining in Vitro and in Silico Single Molecule Force Spectroscopy to Characterize and Tune Cellulosomal Scaffoldin Mechanics
Journal of the American Chemical Society, 139(39): 17841-17852, 2017


Ott, W.#, Jobst, M. A.#, Bauer, M., Durner, E., Milles, L., Nash, M. A., and Gaub. H. E.
Elastin-like Polypeptide Linkers for Single Molecule Force Spectroscopy
ACS Nano, 11 (6): 6346-6354, 2017


Durner, E., Ott, W., Nash, M. A., and Gaub, H. E.
Post-translational Sortase-mediated Attachment of High-strength Force Spectroscopy Handles
ACS Omega, 2(6): 3064-3069, 2017


Ott, W., Jobst, M. A., Schoeler, C., Gaub, H. E., and Nash, M. A.*
Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes: The Current Toolbox
Journal of Structural Biology, 197(1): 3-12, 2017


Milles, L., Bayer, E. A., Nash, M. A., and Gaub, H. E.
Mechanical Stability of a High-Affinity Toxin Anchor From the Pathogen Clostridium Perfringens
Journal of Physical Chemistry B, 121(15): 3620-3626, 2017


Nash, M. A.*
Interfacial Polymerization Systems for Screening of Multi-enzyme Complexes
European Cells and Materials, 32(Supp. 5): 33, 2016

Schoeler, C., Verdorfer, T., Gaub, H. E., and Nash, M. A.*
Biasing Effects of Receptor Ligand Complexes on Protein Unfolding Statistics
Physical Review E, 94: 042412, 2016

Nash, M. A.*, Smith, S. P., Fontes, M. G. A. C., and Bayer, E. A.
Single- versus Dual-binding Conformations in Cellulosomal Cohesin-dockerin Complexes
Current Opinion in Structural Biology, 40:89-96, 2016

Ott, W., Nikolaus, T., Gaub, H. E., and Nash, M. A.*
Sequence Independent Cloning and Post-translational Modification of Repetitive Protein Polymers through Sortase and Sfp-mediated Enzymatic Ligation
Biomacromolecules, 17(4): 1330-1338, 2016

Nash, M. A.* and Shoseyov, O.
Editorial: Nanobiotechnology at a Crossroads: Moving Beyond Proof-of-Concept
Current Opinion in Biotechnology, 39:1-3, 2016

Malinowska, K. H., and Nash, M.A.*
Enzyme- and Affinity Biomolecule-mediated Polymerization Systems for Biological Signal Amplification and Cell Screening
Current Opinion in Biotechnology, 39:68-75, 2016

Gunnoo, M., Cazade, P. A., Galera-Prat, A., Nash, M. A., Czjzek, M., Cieplak, M., Alvarez, B., Aguilar, M., Karpol, A., Gaub, H. E., Carrion-Vazquez, M., Bayer, E. A., and Thompson, D.
Nanoscale Engineering of Designer Cellulosomes
Advanced Materials, 28: 5619-5647, 2016

Jobst, M., Milles, L., Schoeler, C., Ott, W., Fried, D. B., Bayer, E. A., Gaub, H. E., and Nash, M. A.*
Resolving Dual Binding Conformations of Cellulosome Cohesin-Dockerin Complexes using Single-Molecule Force Spectroscopy
eLife, DOI: 10.7554/eLife.10319, 2015

Schoeler, C., Bernardi, R. C., Malinowska, K. M., Durner, E., Ott, W., Bayer, E. A., Schulten, K., Nash, M. A.*, and Gaub, H. E.
Mapping Mechanical Force Propagation Through Biomolecular Complexes
Nano Letters, 15(11): 7370-7376, 2015
[Featured on journal cover; Highlighted on &]

Malinowska, K.H., Rind, T., Verdorfer, T., Gaub, H.E., and Nash, M. A.*
Quantifying Synergy, Thermostability, and Targeting of Cellulolytic Enzymes and Cellulosomes with Polymerization-Based Amplification
Analytical Chemistry, 87(14):7133-7140, 2015

Nash, M. A.*, Bernardi, R., Schoeler, C., Malinowska, K. H., Gaub, H., Schulten, K., and Bayer, E.
Analyzing Force Propagation Through a Stable Protein Complex with Experiments and Simulation
European Biophysical Journal, 44 (Suppl 1): S43–S248, O-307, 2015

Schoeler, C.# Malinowska, K. H.,# Bernardi, R. C., Milles, L. F., Jobst, M. A., Durner, E., Ott, W., Fried, D. B., Bayer, E. A., Schulten, K., Gaub, H. E., and Nash, M. A.*
Ultrastable Cellulosome-Adhesion Complex Tightens Under Load
Nature Communications, 5: 1-35, 2014

Otten, M.,# Ott, W.,# Jobst, M. A.,# Milles, L., Verdorfer, T., Pippig, D., Nash, M. A.*, and Gaub, H.E.
From Genes to Protein Mechanics on a Chip
Nature Methods, 11(11): 1127-1130, 2014
[Highlighted in Nat. Meth. News & Views (subscription required); Cell Press Chemistry & Biology Select]

Malinowska, K., Verdorfer, T., Meinhold, A., Milles, L.F., Funk, V., Gaub, H.E., and Nash, M. A.*
Redox-Initiated Hydrogel System for Detection and Real-Time Imaging of Cellulolytic Enzyme Activity
ChemSusChem, 7(10): 2825-2831, 2014
[Featured on journal cover]

Wolff, M., Braun, D., and Nash, M. A.*
Detection of Thermoresponsive Polymer Phase Transition in Dilute Low-Volume Format by Microscale Thermophoretic Depletion
Analytical Chemistry, 86(14): 6797–6803, 2014
[Featured on journal cover]

Jobst, M. A., Schoeler, C., Malinowska, K., and Nash, M. A.*
Investigating Receptor-Ligand Systems of the Cellulosome using AFM-based Single-molecule Force Spectroscopy
Journal of Visualized Experiments, 82, e50950, 2013

Nash, M. A.*, and Gaub, H. E.
Single-Molecule Adhesion of a Stimuli-Responsive Oligo(ethylene glycol) Co-polymer to Gold
ACS Nano
, 6(12): 10735–10742, 2012
[Featured on journal cover]

Stahl, S. W.#, Nash, M. A.#,*, Fried, D. B, Slutzki, M., Barak, Y., Bayer, E. A., and Gaub, H. E.
Single-Molecule Dissection of the High-Affinity Cohesin-Dockerin Complex
PNAS, 109(50): 20431–20436, 2012

Nash, M. A., Waitumbi, J., Yager, P., Hoffman, A. S., and Stayton, P. S.
Multiplexed Enrichment and Detection of Malarial Biomarkers using a Stimuli-Responsive Iron Oxide and Gold Nanoparticle Reagent System
ACS Nano, 6(8): 6776–6785, 2012

Nash, M. A., Golden, A., Hoffman, J. M., Lai, J. J., and Stayton, P. S.
Smart Surfaces for Point-of-Care Diagnostics
Intelligent Surfaces in Biotechnology. John Wiley & Sons. Grandin, H. M., Textor, M. (Editors) & and Whitesides, G. M. (Foreword), 2012
[Featured on book cover]

Nash, M. A.
Mixed Nanoparticle System for Purification, Enrichment, and Detection of Biomarkers
University of Washington, Seattle – 2011 PhD Dissertation

Nash, M. A.*
Thermal Depolymerization
Green Technology: Green Series Volume 10, 2011 SAGE Publications. Mulvaney, D.R. (Editor)

Nash, M. A.*
Encyclopedia of Nanoscience and Society, 2010 SAGE Publications. Guston, D.H. (Editor)

Nash, M. A., Yager, P., Hoffman, A. S., and Stayton, P. S.
Magnetic Enrichment of “Smart” Gold Nanoparticle-Antibody Conjugates for High-Sensitivity Diagnostic Immunochromatography
European Cells and Materials, 20(SUPPL. 3): 184, 2010

Nash, M. A., Lai, J. J., Hoffman, A. S., Yager, P., and Stayton, P. S.
“Smart” Diblock Copolymers as Templates for Magnetic-Core Gold-Shell Nanoparticle Synthesis
Nano Letters, 10(1): 85–91, 2010

Nash, M. A., Yager, P., Hoffman, A. S., and Stayton, P. S.
Mixed Stimuli-Responsive Magnetic and Gold Nanoparticle System for Rapid Purification, Enrichment, and Detection of Biomarkers
Bioconjugate Chemistry, 21(12): 2197–2204, 2010

Nash, M. A.*, Hoffman, J. M., Stevens, D., Hoffman, A. S., Stayton, P. S., and Yager, P.
Laboratory Scale Protein Striping System for Patterning Biomolecules onto Paper Based Immunochromatographic Test Strips
Lab on a Chip, 10(17): 2279–2282, 2010

Warner, C. L., Addleman, R. S., Cinson, A. D., Droubay, T. C., Engelhard, M. H., Nash, M. A., Yantasee, W., and Warner, M. G.
High Performance Superparamagnetic Nanoparticle Based Heavy Metal Sorbents for Removal of Contaminants from Natural Waters
ChemSusChem, 3(6): 749–757, 2010
[Featured on journal cover]

Lai, J. J., Nelson, K. E., Nash, M. A., Hoffman, A. S., Yager, P., and Stayton, P. S.
Dynamic Bioprocessing and Microfluidic Transport Control with Smart Magnetic Nanoparticles in Laminar-Flow Devices
Lab on a Chip, 9(14): 1997–2002, 2009

Malmstadt, N., Nash, M. A., Purnell, R. F., and Schmidt, J. J.
Automated Formation of Lipid-Bilayer Membranes in a Microfluidic Device
Nano Letters, 6(9): 1961–1965, 2006